Prion Protein(PrP) Recombinant Rabbit Monoclonal Antibody [SC57-05]
cat.: ET1610-27
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: SC57-05
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Prion Protein aa 200-249 / 253.
Positive control: Mouse brain tissue lysate, rat brain tissue lysate, N2A, SHG-44, SH-SY5Y, rat brain tissue, mouse brain tissue, mouse pancreas tissue.
Subcellular location: Golgi apparatus, Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:2,000
1:50-1:200
1:50-1:200
1:50-1:800
1:50-1:100
Uniprot #: SwissProt: P04156 Human | P04925 Mouse | P13852 Rat
Alternative names: Alternative prion protein; major prion protein AltPrP ASCR CD230 CD230 antigen CJD GSS KURU Major prion protein p27 30 PRIO_HUMAN Prion protein Prion related protein PRIP PRNP PrP PrP27 30 PrP27-30 PrP33-35C PrPC PrPSc Sinc
Images
ET1610-27_1.jpg Fig1: Western blot analysis of Prion Protein(PrP) on different lysates with Rabbit anti-Prion Protein(PrP) antibody (ET1610-27) at 1/2,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 20-32 kDa

Exposure time: 3 minutes 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-27) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1610-27_2.jpg Fig2: ICC staining of Prion Protein(PrP) in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-27_3.jpg Fig3: ICC staining of Prion Protein(PrP) in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-27_4.jpg Fig4: ICC staining of Prion Protein(PrP) in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-27, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-27_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Prion Protein(PrP) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-27_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Prion Protein(PrP) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-27_7.jpg Fig7: Flow cytometric analysis of Prion Protein(PrP) was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-27, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1610-27_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Prion Protein(PrP) antibody (ET1610-27) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-27) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.