Phospho-RSK1 (S380) Recombinant Rabbit Monoclonal Antibody [SC05-32]
cat.: ET1610-28
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IP
Clonality: Monoclonal
Clone number: SC05-32
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding S380 of human RSK1.
Positive control: A431 cell lysates, Hela, A431, 293, Hela whole cell lysate, Hela (starved overnight) then treated with TPA (200nM 4h) cell lysate, NIH/3T3 whole cell lysate, NIH/3T3 (starved 24h) then Calyculin A (100nM 15min) cell lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
1:1,000-1:5,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q15418 Human | P18653 Mouse | Q63531 Rat
Alternative names: 90 kDa ribosomal protein S6 kinase 1 dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 dJ590P13.1 EC 2.7.11.1 HU 1 HU1 KS6A1_HUMAN MAP kinase activated protein kinase 1a MAP kinase-activated protein kinase 1a MAPK-activated protein kinase 1a MAPKAP kinase 1a MAPKAPK-1a MAPKAPK1A MGC79981 Mitogen-activated protein kinase-activated protein kinase 1A OTTHUMP00000004113 p90 RSK1 p90-RSK 1 p90rsk p90RSK1 p90S6K pp90RSK1 Ribosomal protein S6 kinase 90kD 1 Ribosomal protein S6 kinase 90kD polypeptide 1 Ribosomal protein S6 kinase 90kDa polypeptide 1 Ribosomal protein S6 kinase alpha 1 Ribosomal protein S6 kinase alpha-1 Ribosomal protein S6 kinase polypeptide 1 Ribosomal S6 kinase 1 RPS6K1 alpha rps6ka Rps6ka1 RSK 1 RSK 1 p90 RSK RSK-1 RSK1 S6K alpha 1 S6K-alpha-1
Images
ET1610-28_1.jpg Fig1: Western blot analysis of Phospho-RSK1 (S380) on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Predicted band size: 83 kDa
Observed band size: 80 kDa
ET1610-28_2.jpg Fig2: ICC staining of Phospho-RSK1 (S380) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-28_3.jpg Fig3: ICC staining of Phospho-RSK1 (S380) in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-28_4.jpg Fig4: ICC staining of Phospho-RSK1 (S380) in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-28_5.jpg Fig5: Western blot analysis of Phospho-RSK1 (S380) on different lysates with Rabbit anti-Phospho-RSK1 (S380) antibody (ET1610-28) at 1/500 dilution.

Lane 1: Hela whole cell lysate
Lane 2: Hela (starved overnight) then treated with TPA (200nM 4h) cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: NIH/3T3 (starved 24h) then Calyculin A (100nM 15min) cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-28) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.