S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02]
cat.: ET1610-3
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Zebrafish, Rat, Goat |
| Applications: | WB, IF-Cell, IF-Tissue, IP, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | SC57-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human S100 beta. |
| Positive control: | Mouse brain tissue, mouse hippocampus tissue, rat brain tissue, mouse liver tissue lysates, SK-MEL-28 cell lysate, rat brain tissue lysate, zebrafish tissue lysates, mouse cerebral cortex tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP IHC-Fr |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:1,000 Use at an assay dependent concentration. 1:100 |
| Uniprot #: | SwissProt: P04271 Human | P50114 Mouse | P04631 Rat |
| Alternative names: | NEF Protein S100 B Protein S100-B S 100 calcium binding protein beta chain S 100 protein beta chain S-100 protein beta chain S-100 protein subunit beta S100 S100 calcium binding protein beta (neural) S100 calcium-binding protein B S100 protein beta chain S100B S100B_HUMAN S100beta |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-S100 beta antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-S100 beta antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-S100 beta antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of S100 beta on mouse liver tissue lysates with Rabbit anti-S100 beta antibody (ET1610-3) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig5:
Western blot analysis of S100 beta on different lysates with Rabbit anti-S100 beta antibody (ET1610-3) at 1/2,000 dilution. Lane 1: SK-MEL-28 cell lysate (15 µg/Lane) Lane 2: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-3) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig6:
Western blot analysis of S100 beta on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Predicted band size: 11 kDa Observed band size: 14 kDa |
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Fig7:
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling S100 beta with Rabbit anti-S100 beta antibody (ET1610-3). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1610-3, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig8:
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling S100 beta with Rabbit anti-S100 beta antibody (ET1610-3). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1610-3, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig9:
Flow cytometric analysis of A375 cells labeling S100 beta. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1610-3, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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