Islet 1 Recombinant Rabbit Monoclonal Antibody [SC05-64]
cat.: ET1610-33
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC05-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Islet 1 aa 262-205 / 349. |
| Positive control: | MCF-7 cell lysates, A549, human thyroid tissue, mouse testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IP IHC-P |
1:500 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. 1:200 |
| Uniprot #: | SwissProt: P61371 Human | P61372 Mouse |
| Alternative names: | Insulin gene enhancer protein ISL 1 Insulin gene enhancer protein ISL-1 Insulin related protein ISL 1 ISL LIM homeobox 1 ISL1 ISL1 transcription factor LIM homeodomain ISL1 transcription factor, LIM/homeodomain (islet 1) ISL1 transcription factor, LIM/homeodomain ISL1_HUMAN Islet-1 Islet1 |
Images
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Fig1:
Western blot analysis of Islet 1 on MCF-7 cell lysates with Rabbit anti-Islet 1 antibody (ET1610-33) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-33) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Islet 1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Islet 1 antibody (ET1610-33) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-33) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Islet 1 antibody (ET1610-33) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-33) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.