Phospho-FAK (Y397) Recombinant Rabbit Monoclonal Antibody [SC54-07]
cat.: ET1610-34
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SC54-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 119 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr397 of human FAK. |
| Positive control: | Hela cell lysate, PC-3 cell lysate, Hela, N2A, NIH/3T3. |
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane, Cell junction. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:500-1:1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q05397 Human | P34152 Mouse | O35346 Rat |
| Alternative names: | FADK 1 FADK FAK related non kinase polypeptide FAK1 FAK1_HUMAN Focal adhesion kinase 1 Focal adhesion Kinase Focal adhesion kinase isoform FAK Del33 Focal adhesion kinase related nonkinase FRNK p125FAK pp125FAK PPP1R71 Protein phosphatase 1 regulatory subunit 71 Protein tyrosine kinase 2 Protein-tyrosine kinase 2 Ptk2 PTK2 protein tyrosine kinase 2 |
Images
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Fig1:
Western blot analysis of Phospho-FAK (Y397) on different lysates with Rabbit anti-Phospho-FAK (Y397) antibody (ET1610-34) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: PC-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-34) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Phospho-FAK (Y397) on Hela cell lysates. Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-FAK (Y397) antibody (ET1610-34) at 1:500 dilution. Anti-Hsp90 antibody (ET1605-56) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 119 kDa Observed band size: 119 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds |
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Fig3: ICC staining of Phospho-FAK (Y397) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of Phospho-FAK (Y397) in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5:
Immunocytochemistry analysis of NIH/3T3 cells labeling Phospho-FAK (Y397) with Rabbit anti-Phospho-FAK (Y397) antibody (ET1610-34) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-FAK (Y397) antibody (ET1610-34) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.