Anti-PI3-kinase p110 subunit beta antibody [SC0580]
cat.: ET1610-36
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IP, IHC-P, FC
Clonality: Monoclonal
Clone number: SC0580
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 123 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PIK3CB aa 5-52 / 1070.
Positive control: Hela cell lysates, human liver carcinoma tissue, Hela.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP

1:1,000
1:50-1:100
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P42338 Human
Alternative names: 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta 5-bisphosphate 3-kinase catalytic subunit beta isoform DKFZp779K1237 MGC133043 OTTHUMP00000216901 OTTHUMP00000216904 p110 BETA p110Beta Phosphatidylinositol 3 kinase catalytic beta polypeptide Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform Phosphatidylinositol-4 Phosphoinositide 3 kinase catalytic beta polypeptide PI3 kinase p110 subunit beta PI3-kinase subunit beta PI3K PI3K beta PI3K-beta PI3Kbeta PI3KCB PIK3C1 Pik3cb PK3CB_HUMAN PtdIns 3 kinase p110 PtdIns-3-kinase subunit beta PtdIns-3-kinase subunit p110-beta
Images
ET1610-36_1.jpg Fig1: Western blot analysis of PI3-kinase p110 subunit beta on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1610-36_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PI3-kinase p110 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-36_3.jpg Fig3: Flow cytometric analysis of PI3-kinase p110 subunit beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-36, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.