PI3-kinase p110 subunit beta Recombinant Rabbit Monoclonal Antibody [SC0580]
cat.: ET1610-36
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | SC0580 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 123 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PIK3CB aa 5-52 / 1070. |
| Positive control: | Jurkat cell lysate, K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, human liver carcinoma tissue, Hela. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:1,000 1:50-1:100 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P42338 Human |
| Alternative names: | 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta 5-bisphosphate 3-kinase catalytic subunit beta isoform DKFZp779K1237 MGC133043 OTTHUMP00000216901 OTTHUMP00000216904 p110 BETA p110Beta Phosphatidylinositol 3 kinase catalytic beta polypeptide Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform Phosphatidylinositol-4 Phosphoinositide 3 kinase catalytic beta polypeptide PI3 kinase p110 subunit beta PI3-kinase subunit beta PI3K PI3K beta PI3K-beta PI3Kbeta PI3KCB PIK3C1 Pik3cb PK3CB_HUMAN PtdIns 3 kinase p110 PtdIns-3-kinase subunit beta PtdIns-3-kinase subunit p110-beta |
Images
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Fig1:
Western blot analysis of PI3-kinase p110 subunit beta on different lysates with Rabbit anti-PI3-kinase p110 subunit beta antibody (ET1610-36) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 123 kDa Observed band size: 110 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PI3-kinase p110 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of PI3-kinase p110 subunit beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-36, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.