Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
Clonality: | Monoclonal |
Clone number: | SC06-14 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 83 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Ku80 aa 693-732 / 732. |
Positive control: | A549, MCF-7, Hela, SW480, human tonsil tissue, human colon cancer tissue, human breast carcinoma tissue, human kideny tissue. |
Subcellular location: | Nucleus, Chromosome. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
Uniprot #: | SwissProt: P13010 Human |
Alternative names: | 86 kDa subunit of Ku antigen ATP dependent DNA helicase 2 subunit 2 ATP dependent DNA helicase II 80 kDa subunit ATP dependent DNA helicase II 86 Kd subunit ATP dependent DNA helicase II ATP-dependent DNA helicase 2 subunit 2 ATP-dependent DNA helicase II 80 kDa subunit CTC box binding factor 85 kDa CTC box-binding factor 85 kDa subunit CTC85 CTCBF DNA repair protein XRCC5 KARP 1 KARP1 Ku 80 Ku autoantigen 80kDa Ku80 Ku86 Ku86 autoantigen related protein 1 KUB 2 KUB2 Lupus Ku autoantigen protein p86 NFIV Nuclear factor IV Thyroid lupus autoantigen Thyroid-lupus autoantigen TLAA X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) X-ray repair cross-complementing protein 5 Xray repair complementing defective repair in Chinese hamster cells 5 XRCC 5 XRCC5 XRCC5_HUMAN |
Fig1:
Western blot analysis of Ku80 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: A549 cell lysate |
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Fig2: ICC staining of Ku80 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig3: ICC staining of Ku80 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig4: ICC staining of Ku80 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Fig5:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Ku80 antibody (ET1610-40) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-40) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human kideny tissue using anti-Ku80 antibody. Counter stained with hematoxylin. Immunohistochemical analysis of paraffin-embedded human kideny tissue with Rabbit anti-Ku80 antibody (ET1610-40) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-40) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Ku80 antibody (ET1610-40) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-40) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig8:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Ku80 antibody (ET1610-40) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-40) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |