p63 Recombinant Rabbit Monoclonal Antibody [SC06-31]
cat.: ET1610-44
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: SC06-31
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 77 kDa
Isotype: IgG
Immunogen: Recombinant protein within human p63 aa 1-250.
Positive control: A431 cell lysates, A431, human tonsil tissue, human skin tissue, human breast tissue, human esophagus tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:800
1:50-1:100
Uniprot #: SwissProt: Q9H3D4 Human | O88898 Mouse | Q9JJP6 Rat
Alternative names: AIS Amplified in squamous cell carcinoma B(p51A) B(p51B) Chronic ulcerative stomatitis protein CUSP DN p63 alpha 1 DNp63 EEC3 id:ibd3516 Keratinocyte transcription factor Keratinocyte transcription factor KET KET LMS MGC115972 MGC192897 NBP OFC8 OTTHUMP00000209732 OTTHUMP00000209733 OTTHUMP00000209734 OTTHUMP00000209735 OTTHUMP00000209737 OTTHUMP00000209738 OTTHUMP00000209739 OTTHUMP00000209740 OTTHUMP00000209741 OTTHUMP00000209742 OTTHUMP00000209743 OTTHUMP00000209744 p40 p51 P51/P63 p53-related protein p63 p53CP p63 P63_HUMAN p73H p73L RHS SHFM4 TAp63alpha TP53CP TP53L TP63 TP73L Transformation related protein 63 Transformation-related protein 63 Trp53rp1 Trp63 Tumor protein 63 Tumor protein p53-competing protein Tumor protein p53-like Tumor protein p63 Tumor protein p63 deltaN isoform delta Tumor protein p73 Tumor protein p73-like
Images
ET1610-44_1.jpg Fig1: Western blot analysis of p63 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1610-44_2.jpg Fig2: ICC staining of p63 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-44, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-44_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p63 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-44_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-p63 antibody (ET1610-44) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-44) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-44_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-p63 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-44_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-p63 antibody (ET1610-44) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-44) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-44_7.jpg Fig7: Flow cytometric analysis of p63 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-44, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.