alpha 1 Catenin Recombinant Rabbit Monoclonal Antibody [SC0619]
cat.: ET1610-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SC0619 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 100 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human alpha 1 Catenin aa 1-90 / 906. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, A431 cell lysate, mouse colon tissue, mouse prostate tissue, mouse uterus tissue, Hela. |
| Subcellular location: | Cytoplasm, Cell junction, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P35221 Human | P26231 Mouse Entrez Gene: 307505 Rat |
| Alternative names: | Alpha E-catenin Cadherin associated protein 102kDa Cadherin associated protein Cadherin-associated protein CAP 102 CAP102 Catenin (cadherin associated protein) alpha 1 102kDa Catenin (cadherin associated protein), alpha 1, 102kDa Catenin alpha 1 Catenin alpha-1 CTNA1_HUMAN CTNNA 1 Ctnna1 FLJ36832 FLJ52416 NY REN 13 antigen OTTHUMP00000224141 OTTHUMP00000224147 Renal carcinoma antigen NY REN 13 Renal carcinoma antigen NY-REN-13 |
Images
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Fig1:
Western blot analysis of alpha 1 Catenin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha 1 Catenin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-alpha 1 Catenin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-alpha 1 Catenin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of alpha 1 Catenin was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-46, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.