MelanA Recombinant Rabbit Monoclonal Antibody [SC56-02]
cat.: ET1610-47
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SC56-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MelanA aa 2-43 / 118. |
| Positive control: | Melanoma tissue lysate, B16F1 cell lysate, human melanoma tissue. |
| Subcellular location: | Golgi apparatus, trans-Golgi network membrane, Endoplasmic reticulum membrane, Melanosome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200 |
| Uniprot #: | SwissProt: Q16655 Human |
| Alternative names: | Antigen LB39 AA Antigen LB39-AA Antigen SK29 AA Antigen SK29-AA MAR1_HUMAN MART 1 MART-1 MART1 Melan A Melan A protein Melanoma antigen recognized by T cells 1 Melanoma antigen recognized by T-cells 1 MLAN A MLANA OTTHUMP00000021036 OTTHUMP00000021037 OTTHUMP00000021038 Protein Melan-A |
Images
|
Fig1:
Western blot analysis of MelanA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Melanoma tissue lysate Lane 2: B16F1 cell lysate |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human melanoma tissue with Rabbit anti-MelanA antibody (ET1610-47) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-47) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.