Asparagine synthetase Recombinant Rabbit Monoclonal Antibody [SC67-07]
cat.: ET1610-50
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SC67-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 64 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Asparagine synthetase aa 502-538 / 561. |
| Positive control: | K562 cell lysate, human skeletal muscle tissue lysate, human lung tissue lysates, Hela, SW480, SH-SY5Y, mouse stomach tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P08243 Human | Q61024 Mouse | P49088 Rat |
| Alternative names: | asnS ASNS_HUMAN ASNSD Asparagine synthetase [glutamine-hydrolyzing] Asparagine synthetase Cell cycle control protein TS11 Glutamine dependent asparagine synthetase 3 Glutamine dependent asparagine synthetase Glutamine hydrolyzing Glutamine-dependent asparagine synthetase OTTHUMP00000024510 OTTHUMP00000204938 OTTHUMP00000204939 OTTHUMP00000204940 OTTHUMP00000204941 OTTHUMP00000204942 TS11 TS11 cell cycle control protein |
Images
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Fig1:
Western blot analysis of Asparagine synthetase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: Human skeletal muscle tissue lysate |
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Fig2:
Western blot analysis of Asparagine synthetase on different lysates with Rabbit anti-Asparagine synthetase antibody (ET1610-50) at 1/500 dilution. Lane 1: mouse pancreas tissue lysate, 20ug Lane 2,3: K562 cell lysate, 10ug. Incubate with different batches of The primary antibody (ET1610-50). Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-50) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of Asparagine synthetase in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of Asparagine synthetase in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: ICC staining of Asparagine synthetase in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Asparagine synthetase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.