ET1610-51

Cardiac Troponin T Recombinant Rabbit Monoclonal Antibody [SC64-03]

cat.: ET1610-51

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Tissue
Clonality:	Monoclonal
Clone number:	SC64-03

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	36 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human Cardiac Troponin T aa 251-298/298.
Positive control:	Mouse heart tissue lysates, mouse heart tissue, rat heart tissue lysates, human striated muscle tissue, rat heart tissue, human heart tissue.
Subcellular location:	Cytoplasm, filament.
Recommended Dilutions: WB IHC-P IF-Tissue	1:500 1:100-1:500 1:500
Uniprot #:	SwissProt: P45379 Human \| P50752 Mouse \| P50753 Rat
Alternative names:	Cardiac muscle troponin T Cardiomyopathy dilated 1D (autosomal dominant) Cardiomyopathy hypertrophic 2 CMD1D CMH2 CMPD2 cTnT LVNC6 MGC3889 OTTHUMP00000033864 OTTHUMP00000033865 OTTHUMP00000033866 OTTHUMP00000033867 OTTHUMP00000033870 OTTHUMP00000218095 RCM3 TNNT 2 TNNT2 TNNT2_HUMAN TnTc Troponin T cardiac muscle Troponin T type 2 (cardiac) Troponin T type 2 cardiac Troponin T, cardiac muscle Troponin T2 cardiac

Images

Fig1: Western blot analysis of Cardiac Troponin T on mouse heart tissue lysates with Rabbit anti-Cardiac Troponin T antibody (ET1610-51) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 40 kDa

Exposure time: 1 minute;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-51) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of Cardiac Troponin T on rat heart tissue lysates with Rabbit anti-Cardiac Troponin T antibody (ET1610-51) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 40/37 kDa

Exposure time: 30 seconds;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-51) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Cardiac Troponin T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-51, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human striated muscle tissue using anti-Cardiac Troponin T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-51, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Cardiac Troponin T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-51, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin T with Rabbit anti-Cardiac Troponin T antibody (ET1610-51) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1610-51, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Fig7: Immunofluorescence analysis of paraffin-embedded mouse heart tissue labeling Cardiac Troponin T with Rabbit anti-Cardiac Troponin T antibody (ET1610-51) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1610-51, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.