Chk2 Recombinant Rabbit Monoclonal Antibody [SC604]
cat.: ET1610-52
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC604 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Chk2 aa 10-200 / 543. |
| Positive control: | A549 cell lysate, HeLa, human testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:1,000-1:2,000 1:50 1:50-1:200 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: O96017 Human |
| Alternative names: | CDS 1 Cds1 Cds1 homolog Checkpoint kinase 2 Checkpoint like protein CHK2 CHEK 2 Chek2 Chk 2 CHK2 checkpoint homolog (S. pombe) CHK2 checkpoint homolog CHK2_HUMAN hCds1 HuCds 1 LFS 2 LFS2 PP1425 RAD 53 RAD53 Rad53 homolog Serine/threonine protein kinase Chk2 Serine/threonine-protein kinase Chk2 |
Images
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Fig1:
Western blot analysis of Chk2 on different lysates with Rabbit anti-Chk2 antibody (ET1610-52) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si Chk2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-52) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling Chk2 with Rabbit anti-Chk2 antibody (ET1610-52) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Chk2 antibody (ET1610-52) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Chk2 antibody (ET1610-52) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Chk2 was immunoprecipitated from 0.2 mg A549 cell lysate with ET1610-52 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1610-52 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: ET1610-52 IP in A549 cell lysate Lane 3: Rabbit IgG instead of ET1610-52 in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.