ATG7 Recombinant Rabbit Monoclonal Antibody [SC06-30]
cat.: ET1610-53
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC06-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ATG7 aa 654-703 / 703. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, THP-1 cell lysate, human tonsil tissue, human spleen tissue, human kidney tissue, human liver tissue, rat kidney tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:1,000-1:2,000 1:50-1:200 1:100-1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O95352 Human | Q641Y5 Rat |
| Alternative names: | 1810013K23Rik Apg 7 APG7 autophagy 7 like APG7 autophagy 7-like (S. cerevisiae) APG7 like APG7, S. cerevisiae, homolog of APG7-like APG7L ATG 7 ATG12-activating enzyme E1 ATG7 ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae) ATG7 autophagy related 7 homolog ATG7_HUMAN Atg7l Autophagy 7, S. cerevisiae, homolog of Autophagy related protein 7 Autophagy-related 7 (yeast) Autophagy-related protein 7 DKFZp434N0735 GSA 7 GSA7 hAGP7 Ubiquitin activating enzyme E1 like protein Ubiquitin-activating enzyme E1-like protein Ubiquitin-like modifier-activating enzyme ATG7 |
Images
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Fig1:
Western blot analysis of ATG7 on different lysates with Rabbit anti-ATG7 antibody (ET1610-53) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 78 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-53) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ATG7 on rat kidney tissue lysates with Rabbit anti-ATG7 antibody (ET1610-53) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 78 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of ATG7 on different lysates with Rabbit anti-ATG7 antibody (ET1610-53) at 1/2,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si ATG7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 78 kDa Observed band size: 70 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-53) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.