Phospho-SMC1 (S957) Recombinant Rabbit Monoclonal Antibody [SC65-03]
cat.: ET1610-55
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: SC65-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 144 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser957 of Human SMC1 aa 945-980 / 1233.
Positive control: HeLa cell lysate, HeLa treated with UV then recovery 30 minutes cell lysate, HeLa cells treated with UV then recovery 30 minutes, human tonsil tissue, human lung adenocarcinoma tissue, human breast carcinoma tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q8NDV3 Human | Q14683 Human
Alternative names: CDLS2 DKFZp686L19178 DXhXs423e DXS423E KIAA0178 MGC138332 OTTHUMP00000061876 RP6 29D12.1 SB1.8 Segregation of mitotic chromosomes 1 Segregation of mitotic chromosomes like 1 SMC 1 SMC protein 1B SMC-1-beta SMC-1B SMC1 SMC1A SMC1alpha SMC1alpha protein SMC1B SMC1B_HUMAN SMC1BETA SMC1beta protein SMC1L1 SMC1L2 SMCB Structural maintenance of chromosome 1 like 1 protein Structural maintenance of chromosome 1 like 2 protein Structural maintenance of chromosomes 1A Structural maintenance of chromosomes 1B Structural maintenance of chromosomes protein 1B
Images
ET1610-55_1.jpg Fig1: Western blot analysis of Phospho-SMC1 (S957) on different lysates with Rabbit anti-Phospho-SMC1 (S957) antibody (ET1610-55) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with UV then recovery 30 minutes cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 144 kDa
Observed band size: 150 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1610-55_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells treated with or without UV then recovery 30 minutes labeling Phospho-SMC1 (S957) with Rabbit anti-Phospho-SMC1 (S957) antibody (ET1610-55) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-SMC1 (S957) antibody (ET1610-55) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1610-55_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-SMC1 (S957) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-55_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using anti-Phospho-SMC1 (S957) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-55, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-55_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-SMC1 (S957) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.