Myoglobin Recombinant Rabbit Monoclonal Antibody [SC06-38]
cat.: ET1610-56
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | SC06-38 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Myoglobin aa 121-154 / 154. |
| Positive control: | Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, C2C12. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IP |
1:1,000-1:5,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P02144 Human | P04247 Mouse | Q9QZ76 Rat |
| Alternative names: | MB MGC13548 MYG_HUMAN Myoglobin PVALB |
Images
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Fig1:
Western blot analysis of Myoglobin on different lysates with Rabbit anti-Myoglobin antibody (ET1610-56) at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-56) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining of Myoglobin in C2C12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-56, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.