Anti-Annexin A10 antibody [SC06-02]
cat.: ET1610-57
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SC06-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 37 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Annexin A10 aa 1-200.
Positive control: Hela cell lysate, Jurkat cell lysate, rat bladder tissue, rat stomach tissue, mouse stomach tissue.
Subcellular location: Cytoplasm, nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:1,000-1:2,000
1:50-1:200
Uniprot #: SwissProt: Q9UJ72 Human | Q9QZ10 Mouse
Unigene: 101584 Rat
Alternative names: annexin 14 Annexin A10 Annexin-10 Annexin-14 ANX 14 ANX10_HUMAN ANX14 ANXA 10 ANXA10
Images
ET1610-57_1.jpg Fig1: Western blot analysis of Annexin A10 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
ET1610-57_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-Annexin A10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-57, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-57_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-Annexin A10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-57, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-57_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Annexin A10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-57, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.