ET1610-6

Sonic Hedgehog Protein/SHH(C-Product) Recombinant Rabbit Monoclonal Antibody [SC05-28]

cat.: ET1610-6

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality:	Monoclonal
Clone number:	SC05-28

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 50 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human Sonic Hedgehog Protein aa 258-307 / 462.
Positive control:	Hela cell lysate, HepG2 cell lysate, Hela, A549, 293, human liver cancer tissue, human kidney tissue, rat kidney tissue, rat liver tissue, mouse kidney tissue lysate, mouse brain tissue lysate, mouse liver tissue lysate, H22 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, human liver tissue, mouse liver tissue.
Subcellular location:	Secreted, Cell membrane.
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P FC	1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:200-1:1,000 1:50-1:100
Uniprot #:	SwissProt: Q15465 Human \| Q62226 Mouse
Alternative names:	HHG 1 HHG-1 HHG1 HLP 3 HLP3 Holoprosencephaly 3 HPE 3 HPE3 MCOPCB5 shh SHH_HUMAN SMMC I SMMCI Sonic Hedgehog (Drosophila) homolog sonic hedgehog homolog (Drosophila) Sonic hedgehog homolog Sonic hedgehog protein Sonic hedgehog protein C-product TPT TPTPS

Images

	Fig1: Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2
	Fig2: ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining Sonic Hedgehog Protein in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: ICC staining Sonic Hedgehog Protein in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

	Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.
	Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.
	Fig8: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

	Fig9: Flow cytometric analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
	Fig10: Western blot analysis of Sonic Hedgehog Protein/SHH(C-Product) on different lysates with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate (30 µg/Lane) Lane 2: Mouse brain tissue lysate (30 µg/Lane) Lane 3: Mouse liver tissue lysate (30 µg/Lane) Lane 4: H22 cell lysate (15 µg/Lane) Lane 5: Neuro-2a cell lysate (15 µg/Lane) Lane 6: NIH/3T3 cell lysate (15 µg/Lane) Predicted band size: 50 kDa Observed band size: 25/55 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-6) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
	Fig11: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig12: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Sonic Hedgehog Protein/SHH(C-Product) antibody (ET1610-6) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.