WASF2 Recombinant Rabbit Monoclonal Antibody [SC66-05]
cat.: ET1610-69
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SC66-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human WASF2 aa 440-485 / 498. |
| Positive control: | mouse kidney tissue, mouse placenta tissue, Hela cell lysate, JK-562 cell lysate. |
| Subcellular location: | Basolateral cell membrane, cytoskeleton, lamellipodium. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9Y6W5 Human | Q8BH43 Mouse Entrez Gene: 313024 Rat |
| Alternative names: | DICTYOSTELIUM dJ393P12.2 IMD2 Protein WAVE-2 Putative Wiskott Aldrich syndrome protein family member 4 SCAR 2 SCAR SCAR2 Suppressor of cyclic-AMP receptor (WASP family) Verprolin homology domain-containing protein 2 WASF2 WASF2_HUMAN WASF4 WASP family protein member 2 WASP family protein member 4 WASP family Verprolin homologous protein 2 WASP-family protein member 2 WAVE 2 WAVE2 Wiskott-Aldrich syndrome protein family member 2 Wiskott-Aldrich syndrome protein family verprolin-homologous protein |
Images
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Fig1:
Western blot analysis of WASF2 on different lysates with Rabbit anti-WASF2 antibody (ET1610-69) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: JK-562 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 72 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1610-69, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 54 kDa Observed band size: 75 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-WASF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-WASF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-WASF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.