Daxx Recombinant Rabbit Monoclonal Antibody [SC54-06]
cat.: ET1610-7
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | SC54-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 81 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Daxx aa 195-313 / 740. |
| Positive control: | PANC-1, A549, Hela, SW480. |
| Subcellular location: | Cytoplasm, Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UER7 Human |
| Alternative names: | BING 2 BING2 CENP-C binding protein DAP 6 DAP6 DAXX DAXX_HUMAN Death associated protein 6 Death domain associated protein 6 Death domain associated protein Death domain-associated protein 6 EAP 1 EAP1 ETS1 associated protein 1 ETS1-associated protein 1 Fas binding protein Fas death domain associated protein Fas death domain-associated protein hDaxx MGC126245 MGC126246 |
Images
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Fig1:
Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A549 Lane 3: SW480 |
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Fig2: ICC staining Daxx in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Daxx in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Flow cytometric analysis of Hela cells with Daxx antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.