MEKK2 Recombinant Rabbit Monoclonal Antibody [SC68-02]
cat.: ET1610-70
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SC68-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 70 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MEKK2 aa 1-47 / 619. |
| Positive control: | SW480, MCF-7, HepG2,HEK293A, MBMEC, mouse brain tissue, human breast carcinoma tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y2U5 Human | Q61083 Mouse Unigene: 213212 Rat |
| Alternative names: | M3K2_HUMAN Map3k2 MAPK/ERK kinase kinase 2 MEK kinase 2 MEKK 2 MEKK2b Mitogen activated protein kinase kinase kinase 2 Mitogen-activated protein kinase kinase kinase 2 |
Images
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Fig1:
All lanes: Western blot analysis of MEKK2 with anti-MEKK2 antibody [SC68-02] (ET1610-70) at 1:1,000 dilution. Lane 1: Wild-type HEK293A whole cell lysate (20 µg). Lane 2: MEKK2 knockout HEK293A whole cell lysate (20 µg). Lane 3: Wild-type MBMEC whole cell lysate (20 µg). Lane 4: MEKK2 knockout MBMEC whole cell lysate (20 µg). ET1610-70 was shown to specifically react with MEKK2 in Wild-type HEK293A and Wild-type MBMEC cells. No band was observed when MEKK2 knockout samples were tested. Wild-type and MEKK2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MEKK2 antibody (ET1610-70, 1/1,000) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Rat brain Lane 3: SW480 |
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Fig3: ICC staining MEKK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining MEKK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining MEKK2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEKK2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MEKK2 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of HepG2 cells with MEKK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.