ATG9A Recombinant Rabbit Monoclonal Antibody [SC67-05]
cat.: ET1610-71
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC67-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 94 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ATG9A aa 799-839 / 839. |
| Positive control: | NCI-H1299 cell lysate, Neuro-2a cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasmic vesicle, Golgi apparatus, Late endosome membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q7Z3C6 Human | Q68FE2 Mouse | Q5FWU3 Rat |
| Alternative names: | APG9 autophagy 9-like 1 APG9 like 1 APG9-like 1 APG9L1 ATG9 ATG9 autophagy related 9 homolog A ATG9 autophagy related 9 homolog A (S. cerevisiae) ATG9A ATG9A_HUMAN Autophagy 9-like 1 protein Autophagy related protein 9A Autophagy-related protein 9A mATG9 MGD3208 OTTHUMP00000206046 OTTHUMP00000206048 OTTHUMP00000206049 OTTHUMP00000206062 |
Images
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Fig1:
All lanes: Western blot analysis of ATG9A with anti-ATG9A antibody [SC67-05] (ET1610-71) at 1:1,000 dilution. Lane 1: Wild-type NCI-H1299 whole cell lysate (20 µg). Lane 2: ATG9A knockout NCI-H1299 whole cell lysate (20 µg). ET1610-71 was shown to specifically react with ATG9A in wild-type NCI-H1299 cells. No band was observed when ATG9A knockout sample was tested. Wild-type and ATG9A knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-71, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ATG9A on different lysates with Rabbit anti-ATG9A antibody (ET1610-71) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 94 kDa Observed band size: 94 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-71) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ATG9A antibody (ET1610-71) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-71) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ATG9A antibody (ET1610-71) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-71) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.