MTCO2 Recombinant Rabbit Monoclonal Antibody [SC06-88]
cat.: ET1610-72
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SC06-88 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MTCO2 aa 199-227 / 227. |
| Positive control: | MCF7 cell lysate, HeLa cell lysate, Human liver tissue lysate, HeLa, human liver tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:2,000 1:100 1:50-1:200 1:200 |
| Uniprot #: | SwissProt: P00403 Human |
| Alternative names: | COII COX2 COX2_HUMAN COXII Cytochrome c oxidase II Cytochrome c oxidase polypeptide II Cytochrome c oxidase subunit 2 MT CO2 MT-CO2 MTCO2 |
Images
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Fig1:
Western blot analysis of MTCO2 on different lysates with Rabbit anti-MTCO2 antibody (ET1610-72) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: Human liver tissue lysate (20 µg/Lane) Predicted band size: 26 kDa Observed band size: 21 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-72) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling MTCO2 with Rabbit anti-MTCO2 antibody (ET1610-72) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MTCO2 antibody (ET1610-72) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MTCO2 antibody (ET1610-72) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-72) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.