MTCO2 Recombinant Rabbit Monoclonal Antibody [SC06-88]
cat.: ET1610-72
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SC06-88 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MTCO2 aa 199-227 / 227. |
| Positive control: | MCF-7 cell lysate, THP-1 cell lysate, Hela cell lysate, human lung tissue lysate, human placenta tissue lysate, HeLa, human liver tissue, HepG2. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:1,000-1:2,000 1:100 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P00403 Human |
| Alternative names: | COII COX2 COX2_HUMAN COXII Cytochrome c oxidase II Cytochrome c oxidase polypeptide II Cytochrome c oxidase subunit 2 MT CO2 MT-CO2 MTCO2 |
Images
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Fig1:
Western blot analysis of MTCO2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: THP-1 cell lysate Lane 3: Hela cell lysate Lane 4: Human lung tissue lysate Lane 5: Human placenta tissue lysate |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling MTCO2 with Rabbit anti-MTCO2 antibody (ET1610-72) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MTCO2 antibody (ET1610-72) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of MTCO2 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-72, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.