Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
Clonality: | Monoclonal |
Clone number: | SC67-06 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 21 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human DIABLO aa 190-239 / 239. |
Positive control: | Jurkat, MCF-7, Hela, B16-F1, LO2, N2A, mouse overy tissue, mouse skin tissue, human spleen tissue, mouse liver tissue. |
Subcellular location: | Mitochondrion. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: Q9NR28 Human | Q9JIQ3 Mouse Unigene: 9090 Rat |
Alternative names: | 0610041G12Rik DBLOH_HUMAN DBOH DFNA64 diablo Diablo homolog (Drosophila) Diablo homolog Diablo homolog mitochondrial Diablo IAP binding mitochondrial protein Diablo like protein DIABLO S Direct IAP binding protein with low pI Direct IAP-binding protein with low pI FLJ10537 FLJ25049 mitochondrial Mitochondrial Smac protein Second mitochondria derived activator of caspase Second mitochondria-derived activator of caspase SMAC 3 Smac Smac protein SMAC3 |
Fig1:
Western blot analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: MCF-7 |
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Fig2: ICC staining DIABLO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining DIABLO in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining DIABLO in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: ICC staining DIABLO in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-DIABLO antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded mouse overy tissue using anti-DIABLO antibody. Counter stained with hematoxylin. | |
Fig8: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DIABLO antibody. Counter stained with hematoxylin. |
Fig9: Flow cytometric analysis of HepG2 cells with DIABLO antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |