DIABLO Recombinant Rabbit Monoclonal Antibody [SC67-06]
cat.: ET1610-74
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SC67-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 21 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DIABLO aa 190-239 / 239.
Positive control: Jurkat, MCF-7, Hela, B16-F1, LO2, N2A, mouse overy tissue, mouse skin tissue, human spleen tissue, mouse liver tissue.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC

1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9NR28 Human | Q9JIQ3 Mouse
Unigene: 9090 Rat
Alternative names: 0610041G12Rik DBLOH_HUMAN DBOH DFNA64 diablo Diablo homolog (Drosophila) Diablo homolog Diablo homolog mitochondrial Diablo IAP binding mitochondrial protein Diablo like protein DIABLO S Direct IAP binding protein with low pI Direct IAP-binding protein with low pI FLJ10537 FLJ25049 mitochondrial Mitochondrial Smac protein Second mitochondria derived activator of caspase Second mitochondria-derived activator of caspase SMAC 3 Smac Smac protein SMAC3
Images
ET1610-74_1.jpg Fig1: Western blot analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: MCF-7
ET1610-74_2.jpg Fig2: ICC staining DIABLO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1610-74_3.jpg Fig3: ICC staining DIABLO in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1610-74_4.jpg Fig4: ICC staining DIABLO in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1610-74_5.jpg Fig5: ICC staining DIABLO in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1610-74_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
ET1610-74_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse overy tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
ET1610-74_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
ET1610-74_9.jpg Fig9: Flow cytometric analysis of HepG2 cells with DIABLO antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.