DIABLO Recombinant Rabbit Monoclonal Antibody [SC67-06]
cat.: ET1610-74
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SC67-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 27 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DIABLO aa 190-239 / 239.
Positive control: HeLa cell lysate, Jurkat cell lysate, C6 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, HeLa, Neuro-2a, C6, human spleen tissue, mouse overy tissue, mouse skin tissue, HepG2.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9NR28 Human | Q9JIQ3 Mouse
Unigene: 9090 Rat
Alternative names: 0610041G12Rik DBLOH_HUMAN DBOH DFNA64 diablo Diablo homolog (Drosophila) Diablo homolog Diablo homolog mitochondrial Diablo IAP binding mitochondrial protein Diablo like protein DIABLO S Direct IAP binding protein with low pI Direct IAP-binding protein with low pI FLJ10537 FLJ25049 mitochondrial Mitochondrial Smac protein Second mitochondria derived activator of caspase Second mitochondria-derived activator of caspase SMAC 3 Smac Smac protein SMAC3
Images
ET1610-74_1.jpg Fig1: Western blot analysis of DIABLO on different lysates with Rabbit anti-DIABLO antibody (ET1610-74) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (40 µg/Lane)
Lane 5: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 27 kDa
Observed band size: 21 kDa

Exposure time: 2 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-74) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1610-74_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling DIABLO with Rabbit anti-DIABLO antibody (ET1610-74) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DIABLO antibody (ET1610-74) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
ET1610-74_3.jpg Fig3: Immunocytochemistry analysis of Neuro-2a cells labeling DIABLO with Rabbit anti-DIABLO antibody (ET1610-74) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DIABLO antibody (ET1610-74) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
ET1610-74_4.jpg Fig4: Immunocytochemistry analysis of C6 cells labeling DIABLO with Rabbit anti-DIABLO antibody (ET1610-74) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DIABLO antibody (ET1610-74) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
ET1610-74_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
ET1610-74_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse overy tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
ET1610-74_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
ET1610-74_8.jpg Fig8: Flow cytometric analysis of HepG2 cells with DIABLO antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.