AKAP14 Recombinant Rabbit Monoclonal Antibody [SC0667]
cat.: ET1610-75
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | SC0667 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human AKAP14 aa 135-170 / 197. |
| Positive control: | HUVEC, Jurkat, Hela, A549. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q86UN6 Human Unigene: 471848 Mouse | O35817 Rat |
| Alternative names: | A kinase (PRKA) anchor protein 14 A kinase anchor protein 14 A kinase anchor protein 28 kDa A-kinase anchoring protein 28 AKAP14 AKAP28 PRKA14 Protein kinase A anchoring protein 14 |
Images
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Fig1:
Western blot analysis of AKAP14 on different lysates using anti-AKAP14 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: A549 |
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Fig2: ICC staining AKAP14 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining AKAP14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining AKAP14 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.