AKAP14 Recombinant Rabbit Monoclonal Antibody [SC0667]
cat.: ET1610-75
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SC0667 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human AKAP14 aa 135-170 / 197. |
| Positive control: | Jurkat cell lysate, HUVEC cell lysate, A549 cell lysate, DU 145 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q86UN6 Human | O35817 Rat Entrez Gene: 434756 Mouse |
| Alternative names: | A kinase (PRKA) anchor protein 14 A kinase anchor protein 14 A kinase anchor protein 28 kDa A-kinase anchoring protein 28 AKAP14 AKAP28 PRKA14 Protein kinase A anchoring protein 14 |
Images
|
Fig1:
Western blot analysis of AKAP14 on different lysates with Rabbit anti-AKAP14 antibody (ET1610-75) at 1/5,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HUVEC cell lysate Lane 3: A549 cell lysate Lane 4: DU 145 cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-75) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.