Anti-Integrin linked ILK antibody [SC68-04]
cat.: ET1610-76
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SC68-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Integrin linked ILK aa 410-452.
Positive control: Hela cell lysate, 293T cell lysate, NIH/3T3, SH-SY5Y, rat brain tissue, Jurkat.
Subcellular location: Cell junction, Cell membrane, Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q13418 Human | O55222 Mouse | Q99J82 Rat
Alternative names: 59 kDa serine/threonine protein kinase 59 kDa serine/threonine-protein kinase DKFZp686F1765 Epididymis secretory protein Li 28 HEL S 28 ILK 1 ILK 2 ILK ILK-1 ILK-2 ILK_HUMAN ILK1 ILK2 Integrin linked kinase 2 Integrin linked Kinase Integrin linked protein kinase Integrin-linked protein kinase p59 p59ILK
Images
ET1610-76_1.jpg Fig1: Western blot analysis of Integrin linked ILK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
ET1610-76_2.jpg Fig2: ICC staining of Integrin linked ILK in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-76_3.jpg Fig3: ICC staining of Integrin linked ILK in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-76, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-76_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Integrin linked ILK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-76, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-76_5.jpg Fig5: Flow cytometric analysis of Integrin linked ILK was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-76, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.