SCF Recombinant Rabbit Monoclonal Antibody [SC61-06]
cat.: ET1610-77
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SC61-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SCF aa 36-85 / 273. |
| Positive control: | Raji cell lysate, COLO205 cell lysate, HT-29 cell lysate, A549 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human prostate cancer tissue, human kidney tissue. |
| Subcellular location: | Cell membrane, Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000-1:2,000 1:50-1:200 1:1:1,000 |
| Uniprot #: | SwissProt: P21583 Human | P20826 Mouse | P21581 Rat |
| Alternative names: | C kit ligand C-kit ligand Ckit ligand DCUA DFNA69 DKFZp686F2250 familial progressive hyperpigmentation 2 FPH2 FPHH KIT ligand Kitl KITLG KL 1 KL1 Mast cell growth factor MGF MGF stem cell factor SCF SCF_HUMAN SF SHEP7 sKITLG Soluble KIT ligand Steel factor steel, mouse, homolog of Stem cell factor Stem cell factor precursor |
Images
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Fig1:
Western blot analysis of SCF on different lysates with Rabbit anti-SCF antibody (ET1610-77) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: COLO205 cell lysate Lane 3: HT-29 cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 35 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of SCF on different lysates with Rabbit anti-SCF antibody (ET1610-77) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 35 kDa Exposure time: 1 minute 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-77) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-SCF antibody (ET1610-77) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SCF antibody (ET1610-77) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.