Mst2 Recombinant Rabbit Monoclonal Antibody [SC05-83]
cat.: ET1610-8
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC05-83 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Mst2. |
| Positive control: | HEK293A cell lysate, CRC cell lysate, HCT116 cell lysate, mouse placenta tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000-1:5,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q13188 Human | Q9JI11 Mouse | O54748 Rat |
| Alternative names: | DKFZp686A2068 FLJ90748 KRS1 KRS2 Mammalian STE20 like protein kinase 1 Mammalian STE20 like protein kinase 2 MST 1 MST 2 MST1 MST2 Serine/threonine kinase 3 (STE20 homolog yeast) Serine/threonine kinase 3 (Ste20 yeast homolog) Serine/threonine kinase 3 Serine/threonine kinase 4 Serine/threonine protein kinase 3 Serine/threonine protein kinase 4 Serine/threonine protein kinase Krs 1 Serine/threonine protein kinase Krs 2 STE20 like kinase MST1 STE20 like kinase MST2 STK 3 STK 4 STK3 STK4 YSK3 |
Images
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Fig1:
All lanes: Western blot analysis of Mst2 with anti-Mst2 antibody [SC05-83] (ET1610-8) at 1:1,000 dilution. Lane 1: Wild-type HEK293A whole cell lysate (20 µg). Lane 2: MST1/2 double knockout HEK293A whole cell lysate (20 µg). ET1610-8 was shown to specifically react with Mst2 in Wild-type HEK293A cells. No band was observed when Mst2 knockout samples were tested. Wild-type and Mst2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Mst2 antibody (ET1610-8, 1/1,000) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Mst2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: CRC cell lysate Lane 2: HCT116 cell lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Mst2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.