Mst2 Recombinant Rabbit Monoclonal Antibody [SC05-83]
cat.: ET1610-8
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: SC05-83
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 56 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human Mst2.
Positive control: HEK293A cell lysate, CRC cell lysate, HCT116 cell lysate, mouse placenta tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  IP

1:1,000-1:5,000
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q13188 Human | Q9JI10 Mouse | O54748 Rat
Alternative names: DKFZp686A2068 FLJ90748 KRS1 KRS2 Mammalian STE20 like protein kinase 1 Mammalian STE20 like protein kinase 2 MST 1 MST 2 MST1 MST2 Serine/threonine kinase 3 (STE20 homolog yeast) Serine/threonine kinase 3 (Ste20 yeast homolog) Serine/threonine kinase 3 Serine/threonine kinase 4 Serine/threonine protein kinase 3 Serine/threonine protein kinase 4 Serine/threonine protein kinase Krs 1 Serine/threonine protein kinase Krs 2 STE20 like kinase MST1 STE20 like kinase MST2 STK 3 STK 4 STK3 STK4 YSK3
Images
ET1610-8_1.jpg Fig1: Western blot analysis of Mst2 on different lysates with Rabbit anti-Mst2 antibody (ET1610-8) at 1/1,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD Mst2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1610-8_2.jpg Fig2: Western blot analysis of Mst2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: CRC cell lysate
Lane 2: HCT116 cell lysate
ET1610-8_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Mst2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.