Anti-APC antibody [SC61-03]
cat.: ET1610-80
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IP
Clonality: Monoclonal
Clone number: SC61-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 160 kDa
Isotype: IgG
Immunogen: Recombinant protein.
Positive control: 293 cell, A431 cell, mouse brain tissue.
Subcellular location: Cytoplasm, Cell junction, Cell membrane.
Recommended Dilutions:
  WB
  ICC

1:1,000-1:5,000
1:50-1:200
Uniprot #: SwissProt: P25054 Human | Q61315 Mouse
Alternative names: Adenomatous Polyposis Coli Adenomatous polyposis coli protein Apc APC_HUMAN CC1 Deleted in polyposis 2.5 DP2 DP2.5 DP3 FAP FPC GS Protein APC
Images
ET1610-80_1.jpg Fig1: Western blot analysis of APC on mouse brain lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1610-80_2.jpg Fig2: ICC staining of APC in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-80, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-80_3.jpg Fig3: ICC staining of APC in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-80, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.