APC Recombinant Rabbit Monoclonal Antibody [SC61-03]
cat.: ET1610-80
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IP, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | SC61-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 312 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human APC aa 51-100 / 2,843. |
| Positive control: | Mouse brain tissue, mouse striatum tissue, rat brain tissue, rat striatum tissue, mouse brain tissue lysates, 293, A431. |
| Subcellular location: | Cytoplasm, Cell junction, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P IHC-Fr |
1:5,000-1:20,000 1:50-1:200 1:200-1:1,000 1:500 |
| Uniprot #: | SwissProt: P25054 Human | Q61315 Mouse | P70478 Rat |
| Alternative names: | Adenomatous Polyposis Coli Adenomatous polyposis coli protein Apc APC_HUMAN CC1 Deleted in polyposis 2.5 DP2 DP2.5 DP3 FAP FPC GS Protein APC |
Images
|
Fig1:
Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-APC antibody (ET1610-80) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1610-80, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig2:
Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-APC antibody (ET1610-80) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1610-80, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Western blot analysis of APC on different lysates with Rabbit anti-APC antibody (ET1610-80) at 1/5,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lane 4: mouse brain tissue lysate Lane 5: rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 160 kDa Observed band size: 160 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-80) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-APC antibody (ET1610-80) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-APC antibody (ET1610-80) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-80) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-APC antibody (ET1610-80) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-80) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-APC antibody (ET1610-80) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig8:
Western blot analysis of APC on different lysates with Rabbit anti-APC antibody (ET1610-80) at 1/5,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lane 4: mouse brain tissue lysate Lane 5: rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 160 kDa Observed band size: 160 kDa Exposure time: 23 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-80) at 1/5,000 dilution was used in antibody dilution buffer (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.