APC Recombinant Rabbit Monoclonal Antibody [SC61-03]
cat.: ET1610-80
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | SC61-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 312 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human APC aa 51-100 / 2,843. |
| Positive control: | mouse brain tissue lysate, 293, A431. |
| Subcellular location: | Cytoplasm, Cell junction, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: P25054 Human | Q61315 Mouse |
| Alternative names: | Adenomatous Polyposis Coli Adenomatous polyposis coli protein Apc APC_HUMAN CC1 Deleted in polyposis 2.5 DP2 DP2.5 DP3 FAP FPC GS Protein APC |
Images
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Fig1: Western blot analysis of APC on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining of APC in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-80, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of APC in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-80, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.