Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | SC06-37 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 62 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human PKR aa 500-540. |
Positive control: | A549 cell lysate, HepG2 cell lysate, MCF-7, human kidney tissue. |
Subcellular location: | Nucleus, Cytoplasm, perinuclear region. |
Recommended Dilutions:
WB |
1:1,000-1:5,000 |
Uniprot #: | SwissProt: P19525 Human |
Alternative names: | Double stranded RNA activated protein kinase; E2AK2_HUMAN eIF-2A protein kinase 2 EIF2AK1 EIF2AK2 Eukaryotic translation initiation factor 2 alpha kinase 2 Eukaryotic translation initiation factor 2-alpha kinase 2 HGNC:9437 Interferon induced double stranded RNA activated protein kinase Interferon inducible elF2 alpha kinase Interferon inducible RNA dependent protein kinase Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase MGC126524 P1/eIF-2A protein kinase P1/eIF2A protein kinase p68 kinase PKR PPP1R83 PRKR Protein kinase interferon inducible double stranded RNA dependent Protein kinase RNA activated Protein kinase RNA-activated Protein phosphatase 1 regulatory subunit 83 Serine/threonine protein kinase TIK Tyrosine protein kinase EIF2AK2 |
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Fig1:
Western blot analysis of PKR on different lysates with Rabbit anti-PKR antibody (ET1610-84) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-84) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |