PKR Recombinant Rabbit Monoclonal Antibody [SC06-37]
cat.: ET1610-84
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: SC06-37
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 62 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PKR aa 500-540.
Positive control: A549 cell lysate, HepG2 cell lysate, MCF-7, human kidney tissue.
Subcellular location: Nucleus, Cytoplasm, perinuclear region.
Recommended Dilutions:
  WB

1:1,000-1:5,000
Uniprot #: SwissProt: P19525 Human
Alternative names: Double stranded RNA activated protein kinase; E2AK2_HUMAN eIF-2A protein kinase 2 EIF2AK1 EIF2AK2 Eukaryotic translation initiation factor 2 alpha kinase 2 Eukaryotic translation initiation factor 2-alpha kinase 2 HGNC:9437 Interferon induced double stranded RNA activated protein kinase Interferon inducible elF2 alpha kinase Interferon inducible RNA dependent protein kinase Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase MGC126524 P1/eIF-2A protein kinase P1/eIF2A protein kinase p68 kinase PKR PPP1R83 PRKR Protein kinase interferon inducible double stranded RNA dependent Protein kinase RNA activated Protein kinase RNA-activated Protein phosphatase 1 regulatory subunit 83 Serine/threonine protein kinase TIK Tyrosine protein kinase EIF2AK2
Images
ET1610-84_1.jpg Fig1: Western blot analysis of PKR on different lysates with Rabbit anti-PKR antibody (ET1610-84) at 1/2,000 dilution.

Lane 1: A549 cell lysate
Lane 2: HepG2 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-84) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.