PKR Recombinant Rabbit Monoclonal Antibody [SC06-37]
cat.: ET1610-84
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC06-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PKR aa 500-540. |
| Positive control: | A549 cell lysate, HepG2 cell lysate, MCF-7, human kidney tissue. |
| Subcellular location: | Nucleus, Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:1,000-1:5,000 1:50-1:100 1:50-1:100 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P19525 Human |
| Alternative names: | Double stranded RNA activated protein kinase; E2AK2_HUMAN eIF-2A protein kinase 2 EIF2AK1 EIF2AK2 Eukaryotic translation initiation factor 2 alpha kinase 2 Eukaryotic translation initiation factor 2-alpha kinase 2 HGNC:9437 Interferon induced double stranded RNA activated protein kinase Interferon inducible elF2 alpha kinase Interferon inducible RNA dependent protein kinase Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase MGC126524 P1/eIF-2A protein kinase P1/eIF2A protein kinase p68 kinase PKR PPP1R83 PRKR Protein kinase interferon inducible double stranded RNA dependent Protein kinase RNA activated Protein kinase RNA-activated Protein phosphatase 1 regulatory subunit 83 Serine/threonine protein kinase TIK Tyrosine protein kinase EIF2AK2 |
Images
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Fig1:
Western blot analysis of PKR on different lysates with Rabbit anti-PKR antibody (ET1610-84) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-84) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of PKR in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-84, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-84, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.