Adiponectin receptor protein 1 Recombinant Rabbit Monoclonal Antibody [SC69-04]
cat.: ET1610-86
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: SC69-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ADIPOR1 aa 5-54 / 375.
Positive control: Hela, HepG2, LO2, human kidney tissue, mouse liver tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q96A54 Human | Q91VH1 Mouse
Unigene: 104556 Rat
Alternative names: ACDCR1 ADIPO R1 Adiponectin receptor protein 1 ADIPOR 1 Adipor1 ADR1_HUMAN CGI 45 CGI 45 protein CGI-45 CGI45 CGI45 protein FLJ25385 FLJ42464 PAQR1 Progestin and adipoQ receptor family member I TESBP1A
Images
ET1610-86_1.jpg Fig1: ICC staining of Adiponectin receptor protein 1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-86, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-86_2.jpg Fig2: ICC staining of Adiponectin receptor protein 1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-86, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-86_3.jpg Fig3: ICC staining of Adiponectin receptor protein 1 in LO2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-86, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-86_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Adiponectin receptor protein 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-86, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-86_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Adiponectin receptor protein 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-86, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-86_6.jpg Fig6: Flow cytometric analysis of Adiponectin receptor protein 1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-86, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.