C/EBP Beta Recombinant Rabbit Monoclonal Antibody [SC55-05]
cat.: ET1610-9
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | SC55-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Rat C/EBP Beta aa 248-297 / 297. |
| Positive control: | PC-12 cell lysates, Hela, MCF-7, PC-12. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB FC IP |
1:500-1:2,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P17676 Human | P28033 Mouse | P21272 Rat |
| Alternative names: | AGP/EBP C EBP beta C/EBP beta C/EBP related protein 2 CCAAT Enhancer Binding Protein beta CCAAT/enhancer-binding protein beta CEBPB CEBPB_HUMAN CRP2 IL 6DBP IL6DBP Interleukin 6 dependent binding protein LAP Liver activator protein Liver enriched transcriptional activator NF IL6 NFIL6 Nuclear factor NF IL6 Nuclear factor NF-IL6 SF B SFB Silencer factor B TCF-5 TCF5 Transcription factor 5 |
Images
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Fig1: Flow cytometric analysis of C/EBP Beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-9, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig2:
Western blot analysis of C/EBP Beta on different lysates with Rabbit anti-C/EBP Beta antibody (ET1610-9) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-87 MG cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 60 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-9) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.