JAM-A Recombinant Rabbit Monoclonal Antibody [SC60-07]
cat.: ET1610-90
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SC60-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human F11R aa 1-100. |
| Positive control: | HT-29 cell lysate, HUVEC cell lysate, TF-1 cell lysate, HeLa cell lysate, Human lung tissue lysate, human breast cancer tissue, human endometrium tissue. |
| Subcellular location: | Cell junction, Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9Y624 Human |
| Alternative names: | CD 321 CD321 CD321 antigen ESTM33 F11 receptor F11R JAM 1 JAM A JAM JAM-1 JAM-A JAM1 JAM1_HUMAN JAMA JCAM Jcam1 Junction adhesion molecule 1 Junction adhesion molecule, mouse, homolog of Junctional adhesion molecule 1 Junctional adhesion molecule A KAT Ly106 PAM 1 PAM-1 PAM1 Platelet adhesion molecule 1 Platelet adhesion molecule Platelet F11 receptor PRO301 UNQ264 |
Images
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Fig1:
Western blot analysis of JAM-A on different lysates with Rabbit anti-JAM-A antibody (ET1610-90) at 1/2,000 dilution. Lane 1: HT-29 cell lysate (20 µg/Lane) Lane 2: HUVEC cell lysate (20 µg/Lane) Lane 3: TF-1 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: Human lung tissue lysate (40 µg/Lane) Predicted band size: 33 kDa Observed band size: 37 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-90) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-JAM-A antibody (ET1610-90) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-90) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-JAM-A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-90, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.