Junctional Adhesion Molecule 1 Recombinant Rabbit Monoclonal Antibody [SC60-07]
cat.: ET1610-90
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SC60-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 33 kDa
Isotype: IgG
Immunogen: Recombinant protein within human F11R aa 1-100.
Positive control: Human liver tissue lysates, mouse liver tissue, mouse brain tissue, human endometrium tissue.
Subcellular location: Cell junction, Cell membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000-1:5,000
1:50-1:200
Uniprot #: SwissProt: Q9Y624 Human | O88792 Mouse | Q9JHY1 Rat
Alternative names: CD 321 CD321 CD321 antigen ESTM33 F11 receptor F11R JAM 1 JAM A JAM JAM-1 JAM-A JAM1 JAM1_HUMAN JAMA JCAM Jcam1 Junction adhesion molecule 1 Junction adhesion molecule, mouse, homolog of Junctional adhesion molecule 1 Junctional adhesion molecule A KAT Ly106 PAM 1 PAM-1 PAM1 Platelet adhesion molecule 1 Platelet adhesion molecule Platelet F11 receptor PRO301 UNQ264
Images
ET1610-90_1.jpg Fig1: Western blot analysis of Junctional Adhesion Molecule 1 on human liver tissue lysates with Rabbit anti-Junctional Adhesion Molecule 1 antibody (ET1610-90) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 37 kDa

Exposure time: 30 seconds;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1610-90_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Junctional Adhesion Molecule 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-90, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-90_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Junctional Adhesion Molecule 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-90, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-90_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-Junctional Adhesion Molecule 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-90, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.