HMBS Recombinant Rabbit Monoclonal Antibody [SC65-07]
cat.: ET1610-91
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SC65-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HMBS aa 1-132 / 299. |
| Positive control: | Hela cell lysate, 293T cell lysate, K562 cell lysates. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P08397 Human | P22907 Mouse | P19356 Rat |
| Alternative names: | HEM3_HUMAN HMBS Hydroxymethylbilane synthase PBG D PBG-D PBGD PORC Porphobilinogen deaminase porphyria, acute; Chester type Pre uroporphyrinogen synthase Pre-uroporphyrinogen synthase UPS Uroporphyrinogen I synthase Uroporphyrinogen I synthetase |
Images
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Fig1:
Western blot analysis of HMBS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate |
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Fig2:
Western blot analysis of HMBS on K562 cell lysates with Rabbit anti-HMBS antibody (ET1610-91) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 41 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-91) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.