NQO2 Recombinant Rabbit Monoclonal Antibody [SC06-68]
cat.: ET1610-92
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SC06-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NQO2 aa 2-49 / 231. |
| Positive control: | Hela cell lysates, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P16083 Human | Q9JI75 Mouse | Q6AY80 Rat |
| Alternative names: | DHQV DIA6 EC 1.10.99.2 MGC94180 NAD(P)H dehydrogenase quinone 2 NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 NAD(P)H menadione oxidoreductase 2 dioxin inducible NMOR2 NQO 2 NQO2 NQO2_HUMAN NRH dehydrogenase [quinone] 2 NRH dehydrogenase NRH:quinone oxidoreductase 2 OTTHUMP00000015948 OTTHUMP00000015949 OTTHUMP00000015953 Ox 2 Ox2 QR2 Quinone Quinone reductase 2 Ribosyldihydronicotinamide dehydrogenase [quinone] Ribosyldihydronicotinamide dehydrogenase |
Images
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Fig1:
Western blot analysis of NQO2 on Hela cell lysates with Rabbit anti-NQO2 antibody (ET1610-92) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-92) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NQO2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-92, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.