NQO2 Recombinant Rabbit Monoclonal Antibody [SC06-68]
cat.: ET1610-92
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SC06-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NQO2 aa 2-49 / 231. |
| Positive control: | Hela cell lysates, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P16083 Human | Q9JI75 Mouse | Q6AY80 Rat |
| Alternative names: | DHQV DIA6 EC 1.10.99.2 MGC94180 NAD(P)H dehydrogenase quinone 2 NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 NAD(P)H menadione oxidoreductase 2 dioxin inducible NMOR2 NQO 2 NQO2 NQO2_HUMAN NRH dehydrogenase [quinone] 2 NRH dehydrogenase NRH:quinone oxidoreductase 2 OTTHUMP00000015948 OTTHUMP00000015949 OTTHUMP00000015953 Ox 2 Ox2 QR2 Quinone Quinone reductase 2 Ribosyldihydronicotinamide dehydrogenase [quinone] Ribosyldihydronicotinamide dehydrogenase |
Images
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Fig1:
Western blot analysis of NQO2 on different lysates with Rabbit anti-NQO2 antibody (ET1610-92) at 1/10,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 120s seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1610-92, 1/10,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 26 kDa Observed band size: 21 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NQO2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-92, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.