NLRP3 Recombinant Rabbit Monoclonal Antibody [SC06-23]
cat.: ET1610-93
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | SC06-23 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 118 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NLRP3 aa 5-161 / 1036. |
| Positive control: | THP-1 cell lysate, RAW264.7 cell lysate, human lung tissue lysates, HUVEC, RAW264.7, human colon carcinoma tissue, human liver carcinoma tissue, mouse bladder tissue, mouse spleen tissue, human lung carcinoma tissue, Jurkat. |
| Subcellular location: | Cytoplasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:100-1:500 1:100-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: Q96P20 Human | Q8R4B8 Mouse Entrez Gene: 287362 Rat |
| Alternative names: | AGTAVPRL AII/AVP Angiotensin/vasopressin receptor AII/AVP like Angiotensin/vasopressin receptor AII/AVP-like C1orf7 Caterpiller protein 1.1 CIAS 1 CIAS1 CLR1.1 Cold autoinflammatory syndrome 1 Cold autoinflammatory syndrome 1 protein Cryopyrin Familial cold autoinflammatory syndrome FCAS FCU LRR and PYD domains-containing protein 3 Muckle-Wells syndrome MWS NACHT NACHT LRR and PYD containing protein 3 NALP 3 NALP3 NALP3_HUMAN NLRP3 PYPAF 1 PYPAF1 PYRIN containing APAF1 like protein 1 PYRIN-containing APAF1-like protein 1 |
Images
|
Fig1:
Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 118 kDa Observed band size: 118 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-93) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of RAW264.7 cells treated with 10μg/mL LPS for 8 hours labeling NLRP3 with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunocytochemistry analysis of HUVEC cells labeling NLRP3 with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-93) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-93) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6: Flow cytometric analysis of NLRP3 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-93, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.