Anti-GAP43 antibody [SC60-06]
cat.: ET1610-94
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SC60-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 25 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human GAP43 aa 189-238 / 238.
Positive control: Mouse brain lysates, mouse cerebellum tissue lysates, Hela, SHG-44, mouse brain tissue, SH-SY5Y.
Subcellular location: Cell membrane, Cell projection, Cell junction.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:500-1:2,000
1:100-1:500
1:100-1:500
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P17677 Human | P06837 Mouse | P07936 Rat
Alternative names: Axonal membrane protein GAP 43 Axonal membrane protein GAP-43 B 50 Calmodulin binding protein P 57 F1 GAP 43 GAP43 Growth Associated Protein 43 Growth-associated protein 43 Nerve Growth Related Peptide Nerve growth related peptide GAP43 NEUM_HUMAN Neural phosphoprotein B 50 Neural phosphoprotein B-50 Neuromodulin Neuron growth associated protein 43 PP46 Protein F1 QtrA-11580 QtrA-13071
Images
ET1610-94_1.jpg Fig1: Western blot analysis of GAP43 on mouse brain lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1610-94_2.jpg Fig2: Western blot analysis of GAP43 on mouse cerebellum tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1610-94_3.jpg Fig3: ICC staining of GAP43 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-94, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-94_4.jpg Fig4: ICC staining of GAP43 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-94, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-94_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GAP43 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-94_6.jpg Fig6: Flow cytometric analysis of GAP43 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-94, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.