Anti-Caspase-9 antibody [SC65-05]
cat.: ET1610-95
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IP
Clonality: Monoclonal
Clone number: SC65-05
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 46/30/17/37 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Caspase-9 aa 110-340.
Positive control: C2C12 cell lysate, Hela cell lysate, Jurkat cell lysate.
Subcellular location: Apoptosome, cytosol, Mitochondrion, nucleus, cytoplasm, protein-containing complex.
Recommended Dilutions:
  WB
  IP

1:500-1:2,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: P55211 Human | Q8C3Q9 Mouse
Alternative names: APAF-3 APAF3 Apoptosis related cysteine peptidase Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 CASP-9 CASP9 CASP9_HUMAN Caspase 9 apoptosis related cysteine peptidase Caspase 9 Dominant Negative Caspase 9c Caspase-9 Caspase-9 subunit p10 ICE LAP6 ICE like apoptotic protease 6 ICE-LAP6 ICE-like apoptotic protease 6 MCH6 PPP1R56 protein phosphatase 1, regulatory subunit 56 RNCASP9
Images
ET1610-95_1.jpg Fig1: Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C2C12 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.