|Product Type:||Recombinant Rabbit monoclonal IgG, primary antibodies|
|Species reactivity:||Human, Mouse|
Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: C2C12 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate