Anti-Hes1 antibody [SC06-21]
cat.: ET1610-97
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: SC06-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 30 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Hes1 aa 200-240.
Positive control: SiHa cell lysate, SK-Br-3 cell lysate, Hela, 293, mouse brain tissue, SH-SY5Y.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:500-1:1,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q14469 Human | P35428 Mouse | Q04666 Rat
Alternative names: bHLHb39 antibody C-HAIRY1 antibody c-hairy1A antibody Class B basic helix-loop-helix protein 39 antibody FLJ20408 antibody Hairy and enhancer of split 1 (Drosophila) antibody Hairy and enhancer of split 1 antibody Hairy homolog (Drosophila) antibody Hairy homolog antibody Hairy like antibody Hairy, Drosophila, homolog of antibody Hairy-like protein antibody Hairy/enhancer of split, Drosophila, homolog of, 1 antibody HAIRY1 antibody HES-1 antibody hes1 antibody Hes1 hairy and enhancer of split 1 (Drosophila) antibody HES1_HUMAN antibody HHL antibody HL antibody HRY antibody MGC129109 antibody OTTHUMP00000209031 antibody RHL antibody Transcription factor HES-1 antibody
Images
ET1610-97_1.jpg Fig1: Western blot analysis of Hes1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-97, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: SK-Br-3 cell lysate
ET1610-97_2.jpg Fig2: ICC staining of Hes1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-97_3.jpg Fig3: ICC staining of Hes1 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-97_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-97_5.jpg Fig5: Flow cytometric analysis of Hes1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-97, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.