COX1/Cyclooxygenase 1 Recombinant Rabbit Monoclonal Antibody [SC68-05]
cat.: ET1610-98
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: SC68-05
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 69 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human COX1 aa 116-159 / 599.
Positive control: NIH/3T3 cell lysate, C2C12 cell lysate, L6 cell lysate, A431 cell lysate, Hela, N2A, C2C12, L6, mouse skin tissue, mouse brain tissue, human breast carcinoma tissue, mouse stomach tissue, human skin tissue.
Subcellular location: Microsome membrane, Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
  IP
1:1,000-1:5,000
1:100-1:500
1:50-1:1,000
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P23219 Human | P22437 Mouse | Q63921 Rat
Alternative names: COX 1 COX 3 COX-1 COX1 Cox3 Cyclooxygenase 1 Cyclooxygenase 3, included Cyclooxygenase-1 EC 1.14.99.1 Partial COX1 proteins, included PCOX1 PGG/HS PGH synthase 1 PGH1_HUMAN PGHS-1 PGHS1 PHS 1 PHS1 Prostaglandin G/H synthase 1 Prostaglandin H2 synthase 1 Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) Prostaglandin-endoperoxide synthase 1 PTGHS PTGS1
Images
ET1610-98_1.jpg Fig1: Western blot analysis of COX1/Cyclooxygenase 1 on different lysates with Rabbit anti-COX1/Cyclooxygenase 1 antibody (ET1610-98) at 1/2,000 dilution.

Lane 1: NIH/3T3 cell lysate
Lane 2: C2C12 cell lysate
Lane 3: L6 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 69 kDa

Exposure time: 2 minutes 18 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-98) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1610-98_2.jpg Fig2: Western blot analysis of COX1/Cyclooxygenase 1 on different lysates with Rabbit anti-COX1/Cyclooxygenase 1 antibody (ET1610-98) at 1/1,000 dilution.

Lane 1: C2C12 cell lysate
Lane 2: A431 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 69 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-98) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1610-98_3.jpg Fig3: All lanes: Western blot analysis of COX1 with anti-COX1/Cyclooxygenase 1 antibody (ET1610-98) at 1/500 dilution.
Lane 1: Wild-type A431 whole cell lysate (10 µg).
Lane 2/3: COX1 knockdown A431 whole cell lysate (10 µg).

ET1610-98 was shown to specifically react with COX1 in wild-type A431 cells. Weakened bands were observed when COX1 knockdown samples were tested. Wild-type and COX1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1610-98_4.jpg Fig4: ICC staining of COX1/Cyclooxygenase 1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-98_5.jpg Fig5: ICC staining of COX1/Cyclooxygenase 1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-98_6.jpg Fig6: ICC staining of COX1/Cyclooxygenase 1 in C2C12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-98_7.jpg Fig7: ICC staining of COX1/Cyclooxygenase 1 in L6 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-98_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-COX1/Cyclooxygenase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-98_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-COX1/Cyclooxygenase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-98_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-COX1/Cyclooxygenase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-98_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-COX1/Cyclooxygenase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-98_12.jpg Fig12: Flow cytometric analysis of COX1/Cyclooxygenase 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-98, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1610-98_13.jpg Fig13: Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-COX1/Cyclooxygenase 1 antibody (ET1610-98) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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