FNTB Recombinant Rabbit Monoclonal Antibody [SC06-44]
cat.: ET1610-99
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB
Clonality: Monoclonal
Clone number: SC06-44
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 49 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human FNTB aa 1-98 / 437.
Positive control: K-562 cell lysate, JAR cell lysate, Rat brain tissue lysate.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: P49356 Human | Q02293 Rat
Alternative names: CAAX farnesyltransferase subunit beta EC 2.5.1.58 EC=2.5.1.58 Farnesyltransferase farnesyltransferase CAAX box beta1 farnesyltransferase, CAAX box, beta FNTB FNTB_HUMAN FPTB FTase beta FTase-beta MGC105303 Protein farnesyltransferase subunit beta RAS proteins prenyltransferase beta Ras proteins prenyltransferase subunit beta
Images
ET1610-99_1.jpg Fig1: Western blot analysis of FNTB on different lysates with Rabbit anti-FNTB antibody (ET1610-99) at 1/5,000 dilution.

Lane 1: K-562 cell lysate (15 µg/Lane)
Lane 2: JAR cell lysate (15 µg/Lane)
Lane 3: Rat brain tissue lysate (30 µg/Lane)

Predicted band size: 49 kDa
Observed band size: 49 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-99) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.