FNTB Recombinant Rabbit Monoclonal Antibody [SC06-44]
cat.: ET1610-99
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SC06-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FNTB aa 1-98 / 437. |
| Positive control: | K562 cell lysates, JAR. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:500 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P49356 Human | Q02293 Rat |
| Alternative names: | CAAX farnesyltransferase subunit beta EC 2.5.1.58 EC=2.5.1.58 Farnesyltransferase farnesyltransferase CAAX box beta1 farnesyltransferase, CAAX box, beta FNTB FNTB_HUMAN FPTB FTase beta FTase-beta MGC105303 Protein farnesyltransferase subunit beta RAS proteins prenyltransferase beta Ras proteins prenyltransferase subunit beta |
Images
|
Fig1:
Western blot analysis of FNTB on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 49 kDa Observed band size: 41 kDa |
|
Fig2: ICC staining of FNTB in JAR cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.