RhoA Recombinant Rabbit Monoclonal Antibody [SN0612]
cat.: ET1611-10
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SN0612 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human aa 144-193 / 193. |
| Positive control: | HUVEC cell lysate, THP-1 cell lysate, HeLa cell lysate, SiHa cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue lysate, PC-12, human liver cancer tissue, mouse brain tissue. |
| Subcellular location: | Cell membrane, Cytoplasm, Cleavage furrow, Midbody, Cell projection. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000-1:2,000 1:50-1:200 1:100 |
| Uniprot #: | SwissProt: P61586 Human | Q9QUI0 Mouse | P61589 Rat |
| Alternative names: | Aplysia ras related homolog 12 ARH12 ARHA H 12 H12 Oncogene RHO H12 Ras homolog family member A Ras homolog gene family member A Rho A Rho cDNA clone 12 RHO H12 RHO12 RHOA RHOA_HUMAN RHOH12 Small GTP binding protein Rho A Transforming protein Rho A Transforming protein RhoA |
Images
|
Fig1:
Western blot analysis of RhoA on different lysates with Rabbit anti-RhoA antibody (ET1611-10) at 1/1,000 dilution. Lane 1: HUVEC cell lysate (20 µg/Lane) Lane 2: THP-1 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: SiHa cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Lane 7: Human brain tissue lysate (40 µg/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-10) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of PC-12 cells labeling RhoA with Rabbit anti-RhoA antibody (ET1611-10) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RhoA antibody (ET1611-10) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-RhoA antibody (ET1611-10) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-10) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RhoA antibody (ET1611-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.