Myosin Light Chain 2 Recombinant Rabbit Monoclonal Antibody [SN67-09]
cat.: ET1611-13
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SN67-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Myosin Light Chain 2 aa 117-166 / 166. |
| Positive control: | Mouse heart tissue lysate, rat heart tissue lysate, hybrid fish (crucian-carp) skin tissue lysates, human fetal skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue, human striated muscle tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:5,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P10916 Human | P51667 Mouse | P08733 Rat |
| Alternative names: | Cardiac myosin light chain-2 Cardiac ventricular myosin light chain 2 CMH10 MLC 2v MLC-2 MLC-2v MLC2 MLRV_HUMAN MYL 2 MYL2 Myosin light chain 2 regulatory cardiac slow Myosin light polypeptide 2 regulatory cardiac slow Myosin regulatory light chain 2 Myosin regulatory light chain 2 ventricular/cardiac muscle isoform Regulatory light chain of myosin RLC of myosin Slow cardiac myosin regulatory light chain 2 ventricular/cardiac muscle isoform |
Images
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Fig1:
Western blot analysis of Myosin Light Chain 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse heart tissue lysate Lane 2: rat heart tissue lysate |
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Fig2: Western blot analysis of Myosin Light Chain 2 on hybrid fish (crucian-carp) skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Myosin Light Chain 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Myosin Light Chain 2 antibody (ET1611-13) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-13) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Myosin Light Chain 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Myosin Light Chain 2 antibody (ET1611-13) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-13) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Myosin Light Chain 2 antibody (ET1611-13) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-13) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Myosin Light Chain 2 was immunoprecipitated in 0.2mg mouse heart lysate with (ET1611-13) at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using (ET1611-13) at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse heart lysate (input) Lane 2: (ET1611-13) IP in mouse heart lysate Lane 3: Rabbit IgG instead of (ET1611-13) in mouse heart lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 15 seconds |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.