IKK alpha Recombinant Rabbit Monoclonal Antibody [SN06-99]
cat.: ET1611-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SN06-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human IKK alpha. |
| Positive control: | HeLa cell lysate, HCT 116 cell lysate, SK-OV-3 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, rat kidney tissue, HeLa. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IP FC |
1:2,000 1:50-1:200 Use at an assay dependent concentration. 1:1,000 |
| Uniprot #: | SwissProt: O15111 Human | Q60680 Mouse Entrez Gene: 309361 Rat |
| Alternative names: | chuk CHUK1 Conserved Helix Loop Helix Ubiquitous Kinase Conserved helix loop ubiquitous kinase Conserved helix-loop-helix ubiquitous kinase I Kappa B Kinase 1 I Kappa B Kinase Alpha I-kappa-B kinase 1 I-kappa-B kinase alpha IkappaB kinase IkB kinase alpha subunit IkBKA IKK 1 IKK A IKK a kinase IKK-A IKK-alpha IKK1 IKKA IKKA_HUMAN Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit Inhibitor of nuclear factor kappa-B kinase subunit alpha NFKBIKA Nuclear Factor Kappa B Inhibitor Kinase Alpha Nuclear factor NF kappa B inhibitor kinase alpha Nuclear factor NF-kappa-B inhibitor kinase alpha Nuclear factor NFkappaB inhibitor kinase alpha Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor TCF-16 TCF16 Transcription factor 16 |
Images
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Fig1:
Western blot analysis of IKK alpha on different lysates with Rabbit anti-IKK alpha antibody (ET1611-15) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (20 µg/Lane) Lane 3: SK-OV-3 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Mouse kidney tissue lysate (40 µg/Lane) Lane 7: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 85 kDa Observed band size: 75 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-15) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-IKK alpha antibody (ET1611-15) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling IKK alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1611-15, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.