IKK alpha Recombinant Rabbit Monoclonal Antibody [SN06-99]
cat.: ET1611-15
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: SN06-99
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 85 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human IKK alpha.
Positive control: Jurkat cell lysate, Hela cell lysate, SW480, mouse skin tissue, Hela.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP

1:1,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: O15111 Human | Q60680 Mouse
Entrez Gene: 309361 Rat
Alternative names: chuk CHUK1 Conserved Helix Loop Helix Ubiquitous Kinase Conserved helix loop ubiquitous kinase Conserved helix-loop-helix ubiquitous kinase I Kappa B Kinase 1 I Kappa B Kinase Alpha I-kappa-B kinase 1 I-kappa-B kinase alpha IkappaB kinase IkB kinase alpha subunit IkBKA IKK 1 IKK A IKK a kinase IKK-A IKK-alpha IKK1 IKKA IKKA_HUMAN Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit Inhibitor of nuclear factor kappa-B kinase subunit alpha NFKBIKA Nuclear Factor Kappa B Inhibitor Kinase Alpha Nuclear factor NF kappa B inhibitor kinase alpha Nuclear factor NF-kappa-B inhibitor kinase alpha Nuclear factor NFkappaB inhibitor kinase alpha Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor TCF-16 TCF16 Transcription factor 16
Images
ET1611-15_1.jpg Fig1: Western blot analysis of IKK alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
ET1611-15_2.jpg Fig2: ICC staining of IKK alpha in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1611-15_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-IKK alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1611-15_4.jpg Fig4: Flow cytometric analysis of IKK alpha was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.