Phospho-Hsp27 (S82) Recombinant Rabbit Monoclonal Antibody [SN06-73]
cat.: ET1611-16
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP
Clonality: Monoclonal
Clone number: SN06-73
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser82 of Human Hsp27 aa 50-99 / 205.
Positive control: Human skeletal muscle tissue lysate, MCF-7 cell lysate.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IP

1:500-1:2,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: P04792 Human | P14602 Mouse | P42930 Rat
Alternative names: Heat shock 27kDa protein 28 kDa heat shock protein CMT2F DKFZp586P1322 epididymis secretory protein Li 102 Estrogen regulated 24 kDa protein Estrogen-regulated 24 kDa protein Heat shock 25kDa protein 1 Heat shock 27 kDa protein Heat shock 27kD protein 1 Heat shock 27kDa protein 1 Heat shock 28kDa protein 1 Heat Shock Protein 27 Heat shock protein beta 1 Heat shock protein beta-1 heat shock protein family B (small) member 1 HEL-S-102 HMN2B HS.76067 Hsp 25 HSP 27 Hsp 28 Hsp B1 Hsp25 HSP27 Hsp28 HspB1 HSPB1_HUMAN SRP27 Stress responsive protein 27 Stress-responsive protein 27
Images
ET1611-16_1.jpg Fig1: Western blot analysis of Phospho-Hsp27 (S82) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: human skeletal muscle tissue lysate
Lane 2: MCF-7 cell lysate
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.