Phospho-MSK1 (S376) Recombinant Rabbit Monoclonal Antibody [SN63-05]
cat.: ET1611-17
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SN63-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 90 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser376 of Human MSK1 aa 351-400 / 802. |
| Positive control: | HEK-293 cells treated with 200nM PMA for 30 minutes, human colon tissue, rat bladder tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P IP |
1:1,000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O75582 Human Unigene: 8940 Rat |
| Alternative names: | 90 kDa ribosomal protein S6 kinase 5 EC 2.7.11.1 KS6A5_HUMAN MGC1911 Mitogen and stress activated protein kinase 1 MSPK1 Nuclear Mitogen And Stress Activated Protein Kinase 1 Nuclear mitogen- and stress-activated protein kinase 1 Ribosomal protein S6 kinase 90kD polypeptide 5 Ribosomal protein S6 kinase 90kDa Ribosomal protein S6 kinase 90kDa polypeptide 5 Ribosomal Protein S6 Kinase Alpha 5 Ribosomal protein S6 kinase alpha-5 RLPK RPS6KA5 RSK Like Protein Kinase RSK-like protein kinase RSKL S6K alpha 5 S6K-alpha-5 |
Images
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Fig1:
Immunocytochemistry analysis of HEK-293 cells treated with or without 200nM PMA for 30 minutes labeling Phospho-MSK1 (S376) with Rabbit anti-Phospho-MSK1 (S376) antibody (ET1611-17) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-MSK1 (S376) antibody (ET1611-17) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Phospho-MSK1 (S376) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-Phospho-MSK1 (S376) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-17, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.