Phospho-RPA32/RPA2 (T21) Recombinant Rabbit Monoclonal Antibody [SN06-36]
cat.: ET1611-18
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SN06-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr21 of Human RPA32 / RPA2 aa 1-50 / 270. |
| Positive control: | HeLa whole cell lysate, HeLa treated with 1uM Camptothecin for 1 hour whole cell lysate, HEK-293 whole cell lysate, HEK-293 treated with UV for 20 minutes then recover 2 hours whole cell lysate, HEK-293 treated with UV for 40 minutes then recover 2 hours whole cell lysate, HeLa treated with UV for 40 minutes then recover 2 hours whole cell lysate, HeLa, Hela cell lysate, HL-60 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IP IF-Cell |
1:1,000 Use at an assay dependent concentration. 1:50 |
| Uniprot #: | SwissProt: P15927 Human | Q62193 Mouse | Q63528 Rat |
| Alternative names: | 60S acidic ribosomal protein P1 AA409079 AI325195 AU020965 ik:tdsubc_2g1 M(2)21C MGC137236 OTTHUMP00000004008 p32 p34 RCJMB04_6d17 replication protein A2, 32kDa REPA2 Replication factor A protein 2 Replication protein A 32 kDa subunit Replication protein A 32kDa subunit Replication protein A 34 kDa subunit Replication protein A Replication Protein A2 (32kDa) Replication protein A2 Replication protein A2, 32kDa RF-A protein 2 Rf-A2 RFA RFA2_HUMAN RP-A p32 RP-A p34 RP21C RPA 2 RPA 32 RPA Rpa2 RPA32 RPA34 RpLP1 RpP2 xx:tdsubc_2g1 zgc:109822 |
Images
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Fig1:
Western blot analysis of Phospho-RPA32/RPA2 (T21) on different lysates with Rabbit anti-Phospho-RPA32/RPA2 (T21) antibody (ET1611-18) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 1uM Camptothecin for 1 hour whole cell lysate Lane 3: HEK-293 whole cell lysate Lane 4: HEK-293 treated with UV for 20 minutes, then recover 2 hours whole cell lysate Lane 5: HEK-293 treated with UV for 40 minutes, then recover 2 hours whole cell lysate Lane 6: HeLa whole cell lysate Lane 7: HeLa treated with UV for 40 minutes, then recover 2 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29/35 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-18) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells treated with or without UV for 40 minutes then recover 2 hours labeling Phospho-RPA32/RPA2 (T21) with Rabbit anti-Phospho-RPA32/RPA2 (T21) antibody (ET1611-18) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-RPA32/RPA2 (T21) antibody (ET1611-18) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Western blot analysis of Phospho-RPA32/RPA2 (T21) on different lysates with Rabbit anti-Phospho-RPA32/RPA2 (T21) antibody (ET1611-18) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa (unphosphorylated) Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-18) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.