Phospho-Hormone sensitive lipase (S853) Recombinant Rabbit Monoclonal Antibody [SN06-39]
cat.: ET1611-19
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SN06-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 117 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser853 of Human LIPE aa 821-870 / 1,076. |
| Positive control: | Rat skeletal muscle tissue lysates, human fetal skeletal muscle tissue, mouse skeletal muscle tissue. |
| Subcellular location: | Cell membrane, cytosol, caveola, Lipid droplet. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q05469 Human | P54310 Mouse | P15304 Rat |
| Alternative names: | Hormone sensitive lipase Hormone sensitive lipase testicular isoform Hormone-sensitive lipase HSL LHS Lipase hormone sensitive LIPE LIPS_HUMAN |
Images
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Fig1:
Western blot analysis of Phospho-Hormone sensitive lipase (S853) on rat skeletal muscle tissue lysates with Rabbit anti-Phospho-Hormone sensitive lipase (S853) antibody (ET1611-19) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 117 kDa Observed band size: 75 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-19) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Phospho-Hormone sensitive lipase (S853) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Phospho-Hormone sensitive lipase (S853) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.