hnRNP C1+C2 Recombinant Rabbit Monoclonal Antibody [SN0652]
cat.: ET1611-2
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SN0652 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of Human HNRNPC aa 60-190 / 306. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, MCF-7 cell lysate, Hela, MCF-7, B16F1, human breast carcinoma tissue, human kidney tissue, mouse brain tissue, mouse skin tissue, mouse placenta tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000-1:5,000 1:100-1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P07910 Human | Q9Z204 Mouse | P17132 Rat |
| Alternative names: | C1 C2 Heterogeneous nuclear ribonucleoprotein C (C1/C2) Heterogeneous nuclear ribonucleoprotein C Heterogeneous nuclear ribonucleoproteins C1/C2 HNRNP hnRNP C1 / hnRNP C2 hnRNP C1/C2 Hnrnpc HNRPC HNRPC_HUMAN MGC104306 MGC105117 MGC117353 MGC131677 Nuclear ribonucleoprotein particle C1 protein Nuclear ribonucleoprotein particle C2 protein SNRPC |
Images
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Fig1:
Western blot analysis of hnRNP C1+C2 on different lysates with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 40 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining hnRNP C1+C2 in B16F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin. |
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Fig10: Flow cytometric analysis of Hela cells with hnRNP C1+C2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.