ET1611-2

hnRNP C1+C2 Recombinant Rabbit Monoclonal Antibody [SN0652]

cat.: ET1611-2

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Zebrafish
Applications:	WB, IF-Tissue, IHC-P, IP, IF-Cell, FC
Clonality:	Monoclonal
Clone number:	SN0652

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 34 kDa
Isotype:	IgG
Immunogen:	Recombinant protein of Human HNRNPC aa 60-190 / 306.
Positive control:	MDA-MB-231 cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat brain tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, Hela, MCF-7, B16F1.
Subcellular location:	Nucleus.
Recommended Dilutions: WB IF-Tissue IHC-P IF-Cell FC	1:2,000-1:5,000 1:100-1:500 1:3,000-8,000 1:100-1:500 1:1,000
Uniprot #:	SwissProt: P07910 Human \| Q9Z204 Mouse \| P17132 Rat
Alternative names:	C1 C2 Heterogeneous nuclear ribonucleoprotein C (C1/C2) Heterogeneous nuclear ribonucleoprotein C Heterogeneous nuclear ribonucleoproteins C1/C2 HNRNP hnRNP C1 / hnRNP C2 hnRNP C1/C2 Hnrnpc HNRPC HNRPC_HUMAN MGC104306 MGC105117 MGC117353 MGC131677 Nuclear ribonucleoprotein particle C1 protein Nuclear ribonucleoprotein particle C2 protein SNRPC

Images

Fig1: Western blot analysis of hnRNP C1+C2 on different lysates with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/2,000 dilution.

Lane 1: MDA-MB-231 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: HepG2 cell lysate (10 µg/Lane)
Lane 4: MCF7 cell lysate (10 µg/Lane)
Lane 5: HL-60 cell lysate (10 µg/Lane)
Lane 6: Mouse brain tissue lysate (20 µg/Lane)

Predicted band size: 34 kDa
Observed band size: 43 kDa

Exposure time: Lane 1-5: 10 seconds; Lane 6: 46 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of hnRNP C1+C2 on different lysates with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: PC-12 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 34 kDa
Observed band size: 43 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1611-2) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1611-2) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-hnRNP C1+C2 antibody (ET1611-2) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1611-2) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: ICC staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

	Fig7: ICC staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig8: ICC staining hnRNP C1+C2 in B16F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig9: Flow cytometric analysis of HeLa cells labeling hnRNP C1+C2. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1611-2, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.