Phospho-SHP2 (Y542) Recombinant Rabbit Monoclonal Antibody [SN61-01]
cat.: ET1611-22
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Human |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | SN61-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr542 of Human SHP2 aa 511-560 / 593. |
| Positive control: | NIH/3T3 treated with 40ng/ml PDGF for 40 minutes whole cell lysate, B16F1. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IP |
1:2,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q06124 Human | P35235 Mouse |
| Alternative names: | BPTP3 CFC JMML METCDS MGC14433 NS1 OTTHUMP00000166107 OTTHUMP00000166108 Protein tyrosine phosphatase 2 Protein tyrosine phosphatase 2C Protein tyrosine phosphatase non receptor type 11 Protein-tyrosine phosphatase 1D Protein-tyrosine phosphatase 2C PTN11_HUMAN PTP-1D PTP-2C PTP1D PTP2C PTPN11 SAP2 SH-PTP2 SH-PTP3 SH2 domain containing protein tyrosine phosphatase 2 SHP 2 SHP-2 Shp2 SHPTP2 SHPTP3 Syp Tyrosine-protein phosphatase non-receptor type 11 |
Images
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Fig1:
Western blot analysis of Phospho-SHP2 (Y542) on different lysates with Rabbit anti-Phospho-SHP2 (Y542) antibody (ET1611-22) at 1/2,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 40ng/mL PDGF for 40 minutes whole cell lysate Lane 3: NIH/3T3 treated with 40ng/mL PDGF for 40 minutes then treated with λpp for 1 hour whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Phospho-SHP2 (Y542) in B16F1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.